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smart cdna library construction kit  (TaKaRa)


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    Structured Review

    TaKaRa smart cdna library construction kit
    Smart Cdna Library Construction Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+cdna+library+construction+kit/SMART+cDNA+Library+Construction+Kit/us12617869-108-20-25
    Average 96 stars, based on 3003 article reviews
    smart cdna library construction kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: Screening of host proteins interacting with the major capsid protein of decapod iridescent virus 1 in Litopenaeus vannamei by the yeast two-hybrid system.
    Article Snippet: Decapod iridescent virus 1 (DIV1) is a fierce pathogen causing high mortality in the decapod crustaceans, including Litopenaeus vannamei.. Major capsid protein (MCP), the main component of the viral capsid, plays a crucial role in iridovirus infection.. To gain insight into the role of MCP in DIV1 infections, a pPR3-N membrane cDNA library derived from L. vannamei hemocyte was established, and the bait plasmid pBT3-STE-MCP was constructed.

    Article Title: MoHTR1, a Nuclear Effector of the Rice Blast Fungus, Regulates Alternative Splicing of Rice Immunity Genes
    Article Snippet: Total RNA was extracted from infected rice leaves according to the supplier’s instructions using Easy-spin total RNA extraction kit (iNtRON Biotechnology). .. We synthesized the cDNA using SMART cDNA library construction kit (Clontech). .. The double stranded cDNA was synthesized and size fractionated using Make your own “Mate & Plate” library system (Clontech).

    cDNA Library Assay:

    Article Title: Screening of host proteins interacting with the major capsid protein of decapod iridescent virus 1 in Litopenaeus vannamei by the yeast two-hybrid system.
    Article Snippet: Decapod iridescent virus 1 (DIV1) is a fierce pathogen causing high mortality in the decapod crustaceans, including Litopenaeus vannamei.. Major capsid protein (MCP), the main component of the viral capsid, plays a crucial role in iridovirus infection.. To gain insight into the role of MCP in DIV1 infections, a pPR3-N membrane cDNA library derived from L. vannamei hemocyte was established, and the bait plasmid pBT3-STE-MCP was constructed.

    Article Title: Molecular diversity of peptides from Pandercetes sp. spider venom and antagonistic activity on voltage-gated calcium channels of the κ-LhTx-1 toxin.
    Article Snippet: The venom of toxic animals represents a rich reservoir of bioactive peptides, which serve as invaluable tools for ion channel research, drug and insecticide development.. While venoms of various species have been extensively studied, the venom composition of spiders from the Pandercetes genus remains largely unexplored.. In this study, the peptide diversity of spider Pandercetes sp. was explored by constructing a venom cDNA library.

    Article Title: Overexpression of BnaMATE43b Improves Resistance to Aluminum Toxicity and Identification of Its Upstream Transcription Factors in Rapeseed ( Brassica napus L.).
    Article Snippet: .. Then, cDNA was reversed using three-frame primers https://doi.org/10.3390/plants15020338 (P1-F/P4-R, P2-F/P4-R, P3-F/P4-R) with the SMART cDNA Library Construction Kit (TaKaRa, Beijing, China). ..

    Article Title: Construction of a Normalized Library and Screening of Transcriptional Regulators of the cas5 Gene in Corynespora cassiicola
    Article Snippet: RNAprep Pure Micro Kit (TianGen, Beijing, China) was used to extract the total RNA of C . cassiicola . .. The library construction method references the SMART cDNA Library Construction Kit (Takara Bio, Mountain View, CA, USA). ..

    Article Title: Genome-wide analysis of the sugarcane PTP family combined with yeast cDNA library screening identifies key cold-responsive genes
    Article Snippet: The quality of RNA was assessed by agarose gel electrophoresis and the RNA concentration was quantified with a NanoDrop2000 spectrophotometer (TIANGEN BIOTECH Co., Ltd., Beijing, China; Cat No. OSE-260). .. The ‘SMART® cDNA Library Construction Kit’ (Takara Biomedical Technology Co., Ltd., Beijing, China; Cat No. 634901) was utilized for reverse transcription and created a sugarcane cDNA library, following the manufacturer’s instructions. ..

    Article Title: Overexpression of BnaMATE43b Improves Resistance to Aluminum Toxicity and Identification of Its Upstream Transcription Factors in Rapeseed ( Brassica napus L.)
    Article Snippet: .. Then, cDNA was reversed using three-frame primers (P1-F/P4-R, P2-F/P4-R, P3-F/P4-R) with the SMART cDNA Library Construction Kit (TaKaRa, Beijing, China). ..

    Article Title: Anti-ADAMTS13 antibody and use thereof
    Article Snippet: .. Specifically, total RNA extracted from the A10 antibody-producing cells was used as a template to perform reverse transcription reaction using SMART CDNA Library Construction Kit (Takara Bio Inc.) capable of amplifying 5′ full-length cDNA in accordance with the attached protocol, to prepare cDNA. .. The cDNA synthesized by the method described above was used as a template with KOD FX (TOYOBO Co., Ltd.) to preform PCR twice (1st PCR and 2nd PCR).

    Article Title: MoHTR1, a Nuclear Effector of the Rice Blast Fungus, Regulates Alternative Splicing of Rice Immunity Genes
    Article Snippet: Total RNA was extracted from infected rice leaves according to the supplier’s instructions using Easy-spin total RNA extraction kit (iNtRON Biotechnology). .. We synthesized the cDNA using SMART cDNA library construction kit (Clontech). .. The double stranded cDNA was synthesized and size fractionated using Make your own “Mate & Plate” library system (Clontech).

    Construct:

    Article Title: Molecular diversity of peptides from Pandercetes sp. spider venom and antagonistic activity on voltage-gated calcium channels of the κ-LhTx-1 toxin.
    Article Snippet: The venom of toxic animals represents a rich reservoir of bioactive peptides, which serve as invaluable tools for ion channel research, drug and insecticide development.. While venoms of various species have been extensively studied, the venom composition of spiders from the Pandercetes genus remains largely unexplored.. In this study, the peptide diversity of spider Pandercetes sp. was explored by constructing a venom cDNA library.

    Isolation:

    Article Title: Molecular diversity of peptides from Pandercetes sp. spider venom and antagonistic activity on voltage-gated calcium channels of the κ-LhTx-1 toxin.
    Article Snippet: The venom of toxic animals represents a rich reservoir of bioactive peptides, which serve as invaluable tools for ion channel research, drug and insecticide development.. While venoms of various species have been extensively studied, the venom composition of spiders from the Pandercetes genus remains largely unexplored.. In this study, the peptide diversity of spider Pandercetes sp. was explored by constructing a venom cDNA library.

    Clone Assay:

    Article Title: Molecular diversity of peptides from Pandercetes sp. spider venom and antagonistic activity on voltage-gated calcium channels of the κ-LhTx-1 toxin.
    Article Snippet: The venom of toxic animals represents a rich reservoir of bioactive peptides, which serve as invaluable tools for ion channel research, drug and insecticide development.. While venoms of various species have been extensively studied, the venom composition of spiders from the Pandercetes genus remains largely unexplored.. In this study, the peptide diversity of spider Pandercetes sp. was explored by constructing a venom cDNA library.

    Plasmid Preparation:

    Article Title: Molecular diversity of peptides from Pandercetes sp. spider venom and antagonistic activity on voltage-gated calcium channels of the κ-LhTx-1 toxin.
    Article Snippet: The venom of toxic animals represents a rich reservoir of bioactive peptides, which serve as invaluable tools for ion channel research, drug and insecticide development.. While venoms of various species have been extensively studied, the venom composition of spiders from the Pandercetes genus remains largely unexplored.. In this study, the peptide diversity of spider Pandercetes sp. was explored by constructing a venom cDNA library.

    Reverse Transcription:

    Article Title: Genome-wide analysis of the sugarcane PTP family combined with yeast cDNA library screening identifies key cold-responsive genes
    Article Snippet: The quality of RNA was assessed by agarose gel electrophoresis and the RNA concentration was quantified with a NanoDrop2000 spectrophotometer (TIANGEN BIOTECH Co., Ltd., Beijing, China; Cat No. OSE-260). .. The ‘SMART® cDNA Library Construction Kit’ (Takara Biomedical Technology Co., Ltd., Beijing, China; Cat No. 634901) was utilized for reverse transcription and created a sugarcane cDNA library, following the manufacturer’s instructions. ..

    Article Title: Anti-ADAMTS13 antibody and use thereof
    Article Snippet: .. Specifically, total RNA extracted from the A10 antibody-producing cells was used as a template to perform reverse transcription reaction using SMART CDNA Library Construction Kit (Takara Bio Inc.) capable of amplifying 5′ full-length cDNA in accordance with the attached protocol, to prepare cDNA. .. The cDNA synthesized by the method described above was used as a template with KOD FX (TOYOBO Co., Ltd.) to preform PCR twice (1st PCR and 2nd PCR).



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